Recombinant Anti-NuMA antibody [EP3976] (ab109262)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3976] to NuMA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NuMA antibody [EP3976]
See all NuMA primary antibodies -
Description
Rabbit monoclonal [EP3976] to NuMA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HeLa, MCF7, Raji, SW480, CACO2, and MOLT4 cell lysates; Human breast carcinoma, normal breast and colonic adenocarcinoma tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3976 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 238 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 238 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
May be a structural component of the nucleus. -
Cellular localization
Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. - Information by UniProt
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Database links
- Entrez Gene: 4926 Human
- Entrez Gene: 101706 Mouse
- Entrez Gene: 308870 Rat
- Omim: 164009 Human
- SwissProt: Q14980 Human
- Unigene: 325978 Human
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Alternative names
- Centrophilin stabilizes mitotic spindle in mitotic cells antibody
- NMP 22 antibody
- Nuclear matrix protein 22 antibody
see all
Images
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ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.
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ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-NuMA antibody [EP3976] (ab109262) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : SW480 cell lysate
Lane 6 : CACO2 cell lysate
Lane 7 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 238 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab109262 has been referenced in 18 publications.
- Wu S et al. A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration. Regen Biomater 9:rbac015 (2022). PubMed: 35529046
- Chaigne A et al. Three-dimensional geometry controls division symmetry in stem cell colonies. J Cell Sci 134:N/A (2021). PubMed: 34323278
- Mercadante DL et al. Modeling reveals cortical dynein-dependent fluctuations in bipolar spindle length. Biophys J 120:3192-3210 (2021). PubMed: 34197801
- Gapud EJ et al. Granzyme B Induces IRF-3 Phosphorylation through a Perforin-Independent Proteolysis-Dependent Signaling Cascade without Inducing Cell Death. J Immunol 206:335-344 (2021). PubMed: 33288544
- Fáberová V et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279