Overview

  • Product name
    Anti-NuMA antibody [EP3976]
    See all NuMA primary antibodies
  • Description
    Rabbit monoclonal [EP3976] to NuMA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NuMA aa 2000-2100. The exact sequence is proprietary.

  • Positive control
    • K562, HeLa, MCF7, Raji, SW480, CACO2, and MOLT4 cell lysates; Human breast carcinoma, normal breast and colonic adenocarcinoma tissues; HeLa cells.
  • General notes

     

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP3976
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 238 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May be a structural component of the nucleus.
    • Cellular localization
      Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
    • Information by UniProt
    • Database links
    • Alternative names
      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    Images

    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.
    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
    • ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.
    • ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • All lanes : Anti-NuMA antibody [EP3976] (ab109262) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Raji cell lysate
      Lane 5 : SW480 cell lysate
      Lane 6 : CACO2 cell lysate
      Lane 7 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 238 kDa

    References

    This product has been referenced in:

    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109262.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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