Recombinant Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5661] to Nuclear Matrix Protein p84
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nuclear Matrix Protein p84 antibody [EPR5661]
See all Nuclear Matrix Protein p84 primary antibodies -
Description
Rabbit monoclonal [EPR5661] to Nuclear Matrix Protein p84 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Nuclear Matrix Protein p84 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, U-87 MG and 293T cell lysates; Human stomach adenocarcinoma tissue; 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5661 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. -
Tissue specificity
Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. -
Sequence similarities
Contains 1 death domain. -
Domain
An intact death domain is needed for apoptosis. -
Post-translational
modificationsExpression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. -
Cellular localization
Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. - Information by UniProt
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Database links
- Entrez Gene: 9984 Human
- Omim: 606930 Human
- SwissProt: Q96FV9 Human
- Unigene: 712543 Human
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Form
Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2). -
Alternative names
- hTREX84 antibody
- Death domain containing protein p84N5 antibody
- HPR 1 antibody
see all
Images
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All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted? -
ab125019, at 1/50 dilution, staining Nuclear Matrix Protein p84 in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab125019, at 1/100 dilution, staining Nuclear Matrix Protein p84 in 293T cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125019 has been referenced in 1 publication.
- Pang G et al. Mitofusin 2 inhibits bladder cancer cell proliferation and invasion via the Wnt/ß-catenin pathway. Oncol Lett 18:2434-2442 (2019). PubMed: 31402945