Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006)
Key features and details
- Mouse monoclonal [NCL/902] to Nucleolin - BSA and Azide free
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nucleolin antibody [NCL/902] - BSA and Azide free
See all Nucleolin primary antibodies -
Description
Mouse monoclonal [NCL/902] to Nucleolin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey, OrangutanDoes not react with: Mouse, Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Human Nucleolin aa 1 to the C-terminus.
Database link: P19338 -
Positive control
- WB: A431 lysate. IHC-P: Human skin, uterus, tonsil, bladder carcinoma, ovarian carcinoma tissues. Flow Cyt: HEK-293T and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NCL/902 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1-2µg for 106 cells.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 76 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use 1-2µg for 106 cells. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 76 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 101865794 Cynomolgus monkey
- Entrez Gene: 4691 Human
- Entrez Gene: 100174230 Orangutan
- Omim: 164035 Human
- SwissProt: Q4R4J7 Cynomolgus monkey
- SwissProt: P19338 Human
- SwissProt: Q5RF26 Orangutan
- Unigene: 79110 Human
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Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
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Anti-Nucleolin antibody [NCL/902] - BSA and Azide free (ab216006) at 1 µg/ml + A431 cell lysate
Predicted band size: 76 kDa -
Flow Cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling Nucleolin with ab216006 at 1 ug/million cells. Grey: Isotype control; Green: FITC-labeled; Purple: APC-labeled; Turquoise: AF700-labeled ab216006.
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Flow Cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling Nucleolin with ab216006 at 1 ug/million cells. Black: cells alone; Grey: Isotype control; Green: AF488-labeled ab216006.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human uterus tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Nucleolin with ab216006 at 1 μg/ml dilution.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab216006 has not yet been referenced specifically in any publications.