Recombinant Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8080-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Entrez Gene: 17975 Mouse
- Entrez Gene: 25135 Rat
- Omim: 164035 Human
- SwissProt: P19338 Human
- SwissProt: P09405 Mouse
- SwissProt: P13383 Rat
- Unigene: 79110 Human
see all -
Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 77 kDa -
ab168363 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab168363 showing +ve staining in Human kidney carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab168363 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab168363 showing +ve staining in Human endometrial carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab168363 has been referenced in 2 publications.
- Luo H et al. Nxhl Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin. Front Cell Dev Biol 9:728821 (2021). PubMed: 34733844
- Huang F et al. Phosphorylation of nucleolin is indispensable to its involvement in the proliferation and migration of non-small cell lung cancer cells. Oncol Rep 41:590-598 (2019). PubMed: 30320343