Anti-OTUD1 antibody (ab122481)
Key features and details
- Rabbit polyclonal to OTUD1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OTUD1 antibody -
Description
Rabbit polyclonal to OTUD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
antigen: SSAAEPVIVS RSDPRDEKLA LYLAEVEKQD KYLRQRNKYR FHIIPDGNCL YRAVSKTVYG DQSLHRELRE QTVHYIADHL DHFSPLIEGD VGEFIIAAAQ D, corresponding to internal sequence amino acids 272-372 of Human OTUD1 (Q5VV17).
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Positive control
- IHC-P: Human lung, lymph node, duodenum, and liver tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 1 OTU domain. - Information by UniProt
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Database links
- Entrez Gene: 220213 Human
- Entrez Gene: 71198 Mouse
- Entrez Gene: 498803 Rat
- Omim: 612022 Human
- SwissProt: Q5VV17 Human
- SwissProt: Q9CUB6 Mouse
- Unigene: 499042 Human
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Alternative names
- DUBA-7 antibody
- DUBA7 antibody
- OTDC1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lung tissue labelling OTUD1 with ab122481 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymph node tissue labelling OTUD1 with ab122481 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of duodenum tissue labelling OTUD1 with ab122481 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling OTUD1 with ab122481 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab122481 has been referenced in 9 publications.
- Ma X et al. The deubiquitinase OTUD1 inhibits non-small cell lung cancer progression by deubiquitinating and stabilizing KLF4. Thorac Cancer 13:761-770 (2022). PubMed: 35098684
- Zhang HG et al. Depression compromises antiviral innate immunity via the AVP-AHI1-Tyk2 axis. Cell Res 32:897-913 (2022). PubMed: 35821088
- Luo Q et al. OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Adv Sci (Weinh) 8:2002874 (2021). PubMed: 33898171
- Song J et al. The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity. EMBO Rep 22:e51162 (2021). PubMed: 33393230
- Chen X et al. OTUD1 Regulates Antifungal Innate Immunity through Deubiquitination of CARD9. J Immunol 206:1832-1843 (2021). PubMed: 33789983
- Yang L et al. Broad Spectrum Deubiquitinase Inhibition Induces Both Apoptosis and Ferroptosis in Cancer Cells. Front Oncol 10:949 (2020). PubMed: 32596160
- Tang Y et al. Alternative Splice Forms of CYLD Mediate Ubiquitination of SMAD7 to Prevent TGFB Signaling and Promote Colitis. Gastroenterology 156:692-707.e7 (2019). PubMed: 30315770
- Zhang L et al. Induction of OTUD1 by RNA viruses potently inhibits innate immune responses by promoting degradation of the MAVS/TRAF3/TRAF6 signalosome. PLoS Pathog 14:e1007067 (2018). PubMed: 29734366
- Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016). IHC . PubMed: 26934049