Recombinant Anti-Orai1 antibody [EPR3394(2)] (ab111960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3394(2)] to Orai1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Orai1 antibody [EPR3394(2)]
See all Orai1 primary antibodies -
Description
Rabbit monoclonal [EPR3394(2)] to Orai1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human platelet lysate and Ovary cancer lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3394(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 35,50 kDa (predicted molecular weight: 33 kDa).
The 50kda band is the glycosylated Orai1 protein (PMID: 26956484). |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 35,50 kDa (predicted molecular weight: 33 kDa). The 50kda band is the glycosylated Orai1 protein (PMID: 26956484). |
Target
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Function
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. -
Involvement in disease
Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be observed. -
Sequence similarities
Belongs to the Orai family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 84876 Human
- Omim: 610277 Human
- SwissProt: Q96D31 Human
- Unigene: 55148 Human
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Alternative names
- Calcium release activated calcium channel protein 1 antibody
- Calcium release activated calcium modulator 1 antibody
- Calcium release-activated calcium channel protein 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab111960 has been referenced in 3 publications.
- Ding C et al. Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection. FASEB J 36:e22468 (2022). PubMed: 35913801
- Salvi A et al. A novel bi-allelic loss-of-function mutation in STIM1 expands the phenotype of STIM1-related diseases. Clin Genet 100:84-89 (2021). PubMed: 33733462
- Zhu M et al. Store-operated Ca(2+) entry regulates glioma cell migration and invasion via modulation of Pyk2 phosphorylation. J Exp Clin Cancer Res 33:98 (2014). PubMed: 25433371