Anti-Otx2 antibody (ab76748)
Key features and details
- Rabbit polyclonal to Otx2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Otx2 antibody
See all Otx2 primary antibodies -
Description
Rabbit polyclonal to Otx2 -
Host species
Rabbit -
Specificity
Reacts specifically with 31 kDa Otx2 protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Rat -
Immunogen
Synthetic peptide derived from the N terminal domain of human Otx2
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Positive control
- This antibody gave a positive siganl in OTX2 Overexpression Lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
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Notes |
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WB
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. -
Tissue specificity
Expressed in brain. -
Involvement in disease
Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures. -
Sequence similarities
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Embryo. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 305858 Rat
- SwissProt: Q64201 Rat
- Unigene: 35222 Rat
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Alternative names
- CPHD6 antibody
- Homeobox protein OTX2 antibody
- MCOPS 5 antibody
see all
Images
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All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml
Lane 1 : OTX1 Recombinant Protein
at 0.1 µg
Lane 2 : OTX2 Overexpression Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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Datasheet download
References (2)
ab76748 has been referenced in 2 publications.
- Moreno-Mármol T et al. Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis. Elife 10:N/A (2021). PubMed: 34545806
- Japp AS et al. High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors. J Neuropathol Exp Neurol 74:110-20 (2015). PubMed: 25575132