Recombinant Anti-PAC-2 antibody [EPR9947(2)] - BSA and Azide free (ab249729)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9947(2)] to PAC-2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PAC-2 antibody [EPR9947(2)] - BSA and Azide free
See all PAC-2 primary antibodies -
Description
Rabbit monoclonal [EPR9947(2)] to PAC-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249729 is the carrier-free version of ab172909.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9947(2) -
Isotype
IgG
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Target
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Function
Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization. -
Tissue specificity
Widely expressed with highest levels in lung, brain and colon. Moderately expressed in muscle, stomach, spleen and heart. Weakly expressed in small intestine, pancreas and liver. Highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. -
Sequence similarities
Belongs to the PSMG2 family. -
Post-translational
modificationsDegraded by the proteasome upon completion of 20S proteasome maturation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56984 Human
- Entrez Gene: 107047 Mouse
- Entrez Gene: 291539 Rat
- Omim: 609702 Human
- SwissProt: Q969U7 Human
- SwissProt: Q9EST4 Mouse
- Unigene: 464652 Human
- Unigene: 150701 Mouse
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Alternative names
- CD40 ligand activated specific transcript 3 antibody
- CLAST3 antibody
- HCCA3 antibody
see all
Images
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All lanes : Anti-PAC-2 antibody [EPR9947(2)] (ab172909) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Predicted band size: 29 kDaThis data was developed using ab172909, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249729 has not yet been referenced specifically in any publications.