Anti-PAPOLA antibody (ab72492)
Key features and details
- Rabbit polyclonal to PAPOLA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAPOLA antibody
See all PAPOLA primary antibodies -
Description
Rabbit polyclonal to PAPOLA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis -
Immunogen
Synthetic peptide within Human PAPOLA aa 650 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P51003 -
Positive control
- WB: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus. -
Sequence similarities
Belongs to the poly(A) polymerase family. -
Post-translational
modificationsPolysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex. -
Cellular localization
Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 10914 Human
- Entrez Gene: 18789 Mouse
- Omim: 605553 Human
- SwissProt: P51003 Human
- SwissProt: Q61183 Mouse
- Unigene: 253726 Human
- Unigene: 255877 Mouse
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Alternative names
- MGC5378 antibody
- PAP alpha antibody
- PAP antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72492 has been referenced in 1 publication.
- Sagawa F et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 30:3994-4005 (2011). PubMed: 21822216