Anti-PAQR5 antibody (ab79517)
Key features and details
- Rabbit polyclonal to PAQR5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAQR5 antibody
See all PAQR5 primary antibodies -
Description
Rabbit polyclonal to PAQR5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide derived from the C terminal of Human PAQR5
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Relevance
PAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 54852 Human
- Entrez Gene: 315741 Rat
- Omim: 607781 Human
- SwissProt: Q9NXK6 Human
- SwissProt: Q5PQT5 Rat
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Alternative names
- FLJ20190 antibody
- Membrane progestin receptor gamma antibody
- mPR gamma antibody
see all
Images
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All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa -
All lanes : Anti-PAQR5 antibody (ab79517) at 500 µg
Lane 1 : HUVEC cell lysate (positive control)
Lanes 2-4 : Non-pregnant ovine caruncle tissue lysate
Lanes 5-7 : Day 30 of gestation ovine caruncle tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa (possible non-specific binding)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab79517 has been referenced in 8 publications.
- Sztachelska M et al. Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological Therapy. J Clin Med 11:N/A (2022). PubMed: 35956024
- Ponikwicka-Tyszko D et al. Mifepristone Treatment Promotes Testicular Leydig Cell Tumor Progression in Transgenic Mice. Cancers (Basel) 12:N/A (2020). PubMed: 33158280
- Ponikwicka-Tyszko D et al. Molecular mechanisms underlying mifepristone's agonistic action on ovarian cancer progression. EBioMedicine 47:170-183 (2019). PubMed: 31466918
- Tocchetti GN et al. Biphasic modulation of cAMP levels by the contraceptive nomegestrol acetate. Impact on P-glycoprotein expression and activity in hepatic cells. Biochem Pharmacol 154:118-126 (2018). WB . PubMed: 29684377
- Jackson AC et al. Progesterone receptor signalling in retinal photoreceptor neuroprotection. J Neurochem 136:63-77 (2016). PubMed: 26447367
- Roche SL et al. Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling. PLoS One 11:e0165197 (2016). PubMed: 27814376
- Coleson MP et al. Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction 150:43-52 (2015). PubMed: 25861798
- Bashour NM & Wray S Progesterone directly and rapidly inhibits GnRH neuronal activity via progesterone receptor membrane component 1. Endocrinology 153:4457-69 (2012). PubMed: 22822163