Anti-PAX8 antibody [PAX8R1] (ab53490)
Key features and details
- Mouse monoclonal [PAX8R1] to PAX8
- Suitable for: Flow Cyt, IHC-P, WB, IP, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PAX8 antibody [PAX8R1]
See all PAX8 primary antibodies -
Description
Mouse monoclonal [PAX8R1] to PAX8 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, IP, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PAX8 (C terminal).
Database link: Q06710 -
Positive control
- HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells
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General notes
IHC-P is not batch-tested and therefore variability between batches might be seen for these applications.
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
PAX8R1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
0.5-2 µg/sample
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa.
|
|
IP |
Use at an assay dependent concentration.
1-5 µg/sample |
|
ICC |
Use a concentration of 2 - 100 µg/ml.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. 0.5-2 µg/sample
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa. |
IP
Use at an assay dependent concentration. 1-5 µg/sample |
ICC
Use a concentration of 2 - 100 µg/ml. |
Target
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Function
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. -
Tissue specificity
Expressed in the excretory system, thyroid gland and Wilms tumors. -
Involvement in disease
Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. -
Sequence similarities
Contains 1 paired domain. -
Developmental stage
In developing excretory system, during thyroid differentiation and in adult thyroid. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7849 Human
- Entrez Gene: 18510 Mouse
- Entrez Gene: 81819 Rat
- Omim: 167415 Human
- SwissProt: Q06710 Human
- SwissProt: Q00288 Mouse
- SwissProt: P51974 Rat
- Unigene: 469728 Human
see all -
Alternative names
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
Images
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HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)
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IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
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Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab53490 has been referenced in 32 publications.
- Zang J et al. MiR-326 inhibits trophoblast growth, migration, and invasion by targeting PAX8 via Hippo pathway. Reprod Biol Endocrinol 20:38 (2022). PubMed: 35209928
- Zhang YL et al. Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells. Bioengineered 13:6729-6739 (2022). PubMed: 35246011
- Hsu CF et al. Ovulation Enhances Intraperitoneal and Ovarian Seedings of High-Grade Serous Carcinoma Cells Originating from the Fallopian Tube: Confirmation in a Bursa-Free Mouse Xenograft Model. Int J Mol Sci 23:N/A (2022). PubMed: 35682896
- Goea L et al. Hnf1b renal expression directed by a distal enhancer responsive to Pax8. Sci Rep 12:19921 (2022). PubMed: 36402859
- Uchida A et al. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Nat Commun 13:7860 (2022). PubMed: 36543770