Anti-PCF11 antibody (ab134391)
Key features and details
- Rabbit polyclonal to PCF11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCF11 antibody
See all PCF11 primary antibodies -
Description
Rabbit polyclonal to PCF11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1505-1555 of Human PCF11 (NP_056969.2).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 173 kDa.
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IP |
Use a concentration of 2 - 10 µg/ml.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 173 kDa. |
IP
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
Component of pre-mRNA cleavage complex II. -
Sequence similarities
Contains 1 CID domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51585 Human
- Omim: 608876 Human
- SwissProt: O94913 Human
- Unigene: 128959 Human
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Alternative names
- hPCF11 antibody
- KIAA0824 antibody
- OTTHUMP00000234826 antibody
see all
Images
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All lanes : Anti-PCF11 antibody (ab134391) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 173 kDa
Exposure time: 30 seconds -
Detection of PCF11 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab134391 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134391 has been referenced in 2 publications.
- Kamieniarz-Gdula K et al. Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination. Mol Cell 74:158-172.e9 (2019). PubMed: 30819644
- Volanakis A et al. WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription. Genes Dev 31:2175-2185 (2017). PubMed: 29196535