Anti-PCK1/PEPC antibody (ab70358)
Key features and details
- Rabbit polyclonal to PCK1/PEPC
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-PCK1/PEPC antibody
See all PCK1/PEPC primary antibodies -
Description
Rabbit polyclonal to PCK1/PEPC -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human PCK1/PEPC protein (ab119469) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HepG2 Whole Cell Lysate Rat Liver Tissue Lysate Mouse Liver Tissue Lysate Human Liver Tissue Lysate - adult normal tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Abcam recommends using milk as the blocking agent. |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). Abcam recommends using milk as the blocking agent. |
Target
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Function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
Tissue specificity
Major sites of expression are liver, kidney and adipocytes. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (cytosolic PEPCK deficiency) [MIM:261680]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
Post-translational
modificationsAcetylation is increased on addition of glucose and appears to regulate the protein stability. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5105 Human
- Entrez Gene: 18534 Mouse
- Entrez Gene: 362282 Rat
- Omim: 614168 Human
- SwissProt: P35558 Human
- SwissProt: Q9Z2V4 Mouse
- SwissProt: P07379 Rat
- Unigene: 1872 Human
see all -
Alternative names
- cytosolic [GTP] antibody
- GTP antibody
- PCK1 antibody
see all
Images
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All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 32 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk before being incubated with ab70358 overnight at 4°C. Antibody binding was detected using an anti-rabbit VHH single domain antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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All lanes : Anti-PCK1/PEPC antibody (ab70358) at 1 µg/ml
All lanes : Mouse adipose tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 5 hours
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab70358 has been referenced in 26 publications.
- Yuan Q et al. Sirt1 Mediates Vitamin D Deficiency-Driven Gluconeogenesis in the Liver via mTorc2/Akt Signaling. J Diabetes Res 2022:1755563 (2022). PubMed: 35132380
- Peng J et al. Activation of the canonical ER stress IRE1-XBP1 pathway by insulin regulates glucose and lipid metabolism. J Biol Chem 298:102283 (2022). PubMed: 35863429
- Larocque JC et al. Sexual dimorphism in response to repetitive bouts of acute aerobic exercise in rodents with type 1 diabetes mellitus. PLoS One 17:e0273701 (2022). PubMed: 36083870
- Fujimoto M et al. Liver group 2 innate lymphoid cells regulate blood glucose levels through IL-13 signaling and suppression of gluconeogenesis. Nat Commun 13:5408 (2022). PubMed: 36109558
- Xu XH et al. Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway. Curr Med Sci 40:530-538 (2020). PubMed: 32681256