Anti-PCNA antibody [5E6/2] (ab70472)
Key features and details
- Mouse monoclonal [5E6/2] to PCNA
- Suitable for: IP, ELISA, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-PCNA antibody [5E6/2]
See all PCNA primary antibodies -
Description
Mouse monoclonal [5E6/2] to PCNA -
Host species
Mouse -
Tested applications
Suitable for: IP, ELISA, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae PCNA. Purified recombinant full length his tagged PCNA from Saccharomyces cerevisiae, produced in Escherichia coli.
Database link: P15873 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5E6/2 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. |
Target
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Function
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
Sequence similarities
Belongs to the PCNA family. -
Post-translational
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
Cellular localization
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
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Database links
- Entrez Gene: 852385 Saccharomyces cerevisiae
- SwissProt: P15873 Saccharomyces cerevisiae
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Alternative names
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab70472 has been referenced in 15 publications.
- Brown RE et al. The RNA export and RNA decay complexes THO and TRAMP prevent transcription-replication conflicts, DNA breaks, and CAG repeat contractions. PLoS Biol 20:e3001940 (2022). PubMed: 36574440
- Reyes GX et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Curr Biol 31:1268-1276.e6 (2021). PubMed: 33417883
- Guilliam TA & Yeeles JTP Reconstitution of translesion synthesis reveals a mechanism of eukaryotic DNA replication restart. Nat Struct Mol Biol 27:450-460 (2020). PubMed: 32341533
- Kulkarni DS et al. PCNA activates the MutL? endonuclease to promote meiotic crossing over. Nature 586:623-627 (2020). PubMed: 32814343
- Paul Solomon Devakumar LJ et al. Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex. Nucleic Acids Res 47:6826-6841 (2019). PubMed: 31114918