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Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
Database link: Q9NZQ7
Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565)
Additional information on positive controls:
Positives: B-CPAP- high, ES-2- medium, HCC70 - low
For primary negative control, isotype control, RabMAb negative control antibody (ab172730) is recommended.
For negative control sample, cell line COLO205 is recommended.
For PD-L1 protein, see ab167713.
For recommended Flow Cytometry (Flow Cyt) protocol, please refer to the protocol book (line 1) in the protocol section and/or here (downloadable copy)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab209962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab209478 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
The cellular localisation of this product has been verified in ICC/IF.
Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209962.
The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209962, 1/100 dilution) for 30 min at 4ºC.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/42 bandpass filter.
ab209962 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"