Overview

  • Product name
  • Description
    Rabbit polyclonal to PDCD5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 21 amino acid synthetic peptide from near the carboxy terminus of Human PDCD5.

  • Positive control
    • EL4 cell lysate. Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab83958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

Images

  • Anti-PDCD5 antibody (ab83958) at 1 µg/ml + EL4 cell lysate at 15 µg

    Predicted band size: 14 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry of PDCD5 in Jurkat cells using ab83958 at 2ug/ml.
  • Immunofluorescence of PDCD5 in Jurkat cells using ab83958 at 10 ug/ml.

References

ab83958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Tissue lysate - nuclear (HEK 293 T)
Total protein in input
100 µg
Specification
HEK 293 T
Immuno-precipitation step
Protein A/G

Dr. Chris Tracy

Verified customer

Submitted Sep 26 2012

Answer

DISCOUNT CODE: XXXXX Expiration date: XX-XX-XX   I am very pleased to hear you would like to accept our offer and test ab83958 in ChIP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.   Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:  1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.   Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.   The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Immunoprecipitation
Sample
Rabbit Cell lysate - whole cell (HEK 293T and U2OS)
Total protein in input
1000 µg
Specification
HEK 293T and U2OS
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
10 µg
Specification
HEK 293T
Treatment
Negative Control or PDCD5 siRNA for 96 hrs
Gel Running Conditions
Reduced Denaturing (16.5% acrylamide, tricine)
Blocking step
LiCor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 Cells)
Loading amount
10 µg
Specification
HEK293 Cells
Gel Running Conditions
Reduced Denaturing (16% Tricine)
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

Dr. Chris Tracy

Verified customer

Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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