Anti-PDE1A antibody (ab96336)
Key features and details
- Rabbit polyclonal to PDE1A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE1A antibody
See all PDE1A primary antibodies -
Description
Rabbit polyclonal to PDE1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Recombinant fragment corresponding to a region within amino acids 212-520 of Human PDE1A (NP_001003683).
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Positive control
- EB: H1299 and HeLa whole cell lysates; 293T, A431, H1299, HepG2, MOLT4 and Raji cell lysates ICC/If: HeLa cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. -
Tissue specificity
Several tissues, including brain, kidney, testes and heart. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 281969 Cow
- Entrez Gene: 403652 Dog
- Entrez Gene: 5136 Human
- Entrez Gene: 18573 Mouse
- Entrez Gene: 81529 Rat
- GenBank: NP_005010.2 Human
- Omim: 171890 Human
- SwissProt: P14100 Cow
see all -
Alternative names
- 3' 5' cyclic nucleotide phosphodiesterase antibody
- 5''-cyclic nucleotide phosphodiesterase 1A antibody
- 61 kDa Cam PDE antibody
see all
Images
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All lanes : Anti-PDE1A antibody (ab96336) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS-PAGE -
ab96336, at a 1/200 dilution, staining PDE1A in paraformaldehyde fixed HeLa by Immunofluorescence.
Lower image is merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96336 has been referenced in 1 publication.
- Li L et al. Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. Am J Physiol Heart Circ Physiol 302:H2112-21 (2012). WB . PubMed: 22447941