Anti-PDE4D3 antibody (ab14614)
Key features and details
- Rabbit polyclonal to PDE4D3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE4D3 antibody -
Description
Rabbit polyclonal to PDE4D3 -
Host species
Rabbit -
Specificity
ab14614 does not cross react with PDE4D1, D2, D4 and D5. Does not cross react with PDE4A, PDE4B or PDE4C. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to PDE4D3 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 93 kDa.
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Notes |
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WB
1/500. Detects a band of approximately 93 kDa. |
Target
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Relevance
Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 5144 Human
- Omim: 600129 Human
- SwissProt: Q08499 Human
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Alternative names
- DPDE3 antibody
- hPDE4D3 antibody
- HSPDE4D antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab14614 has been referenced in 3 publications.
- Peng S et al. Phosphodiesterase 4 Inhibitor Roflumilast Protects Rat Hippocampal Neurons from Sevoflurane Induced Injury via Modulation of MEK/ERK Signaling Pathway. Cell Physiol Biochem 45:2329-2337 (2018). PubMed: 29550808
- Rababa'h A et al. Protein kinase A and phosphodiesterase-4D3 binding to coding polymorphisms of cardiac muscle anchoring protein (mAKAP). J Mol Biol 425:3277-88 (2013). PubMed: 23806656
- Raymond DR et al. Distinct phosphodiesterase-4D variants integrate into protein kinase A-based signaling complexes in cardiac and vascular myocytes. Am J Physiol Heart Circ Physiol 296:H263-71 (2009). WB ; Human . PubMed: 19060129