Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)
- Datasheet
- References (117)
- Protocols
Overview
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Product nameAnti-PDGFR beta antibody [Y92] - C-terminal
See all PDGFR beta primary antibodies -
DescriptionRabbit monoclonal [Y92] to PDGFR beta - C-terminal
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Host speciesRabbit
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SpecificityThis antibody recognizes human platelet-derived growth factor (PDGF) receptor beta. It does not cross-react with other CSF-1/PDGF receptor family members. Expression levels of the target protein vary with sample type and some optimisation may be required.
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Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-FoFr, WB, IHC-P, ICC/IF, IP, IHC-FrFlmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human PDGFR beta aa 1050 to the C-terminus. The exact sequence is proprietary.
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Positive control
- WB: NIH/3T3 cell lysate. SH-SY5Y cell lysate. Rat brain and heart tissue lysate. Mouse brain tissue lysate. Human fetal brain tissue lysate. ICC/IF: NIH/3T3 cells. IHC-P: Human prostatic carcinoma, lung cancer, breast and spleen tissue. Flow Cyt: NIH/3T3 cells. IP: NIH/3T3 cell lysate.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberY92
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-PDGFR beta antibody [Y92] (HRP) (ab195515)
- Anti-PDGFR beta antibody [Y92] (Alexa Fluor® 488) (ab196376)
- Anti-PDGFR beta antibody [Y92] (Alexa Fluor® 594) (ab206872)
- Anti-PDGFR beta antibody [Y92] (Alexa Fluor® 405) (ab206873)
- Anti-PDGFR beta antibody [Y92] (Alexa Fluor® 555) (ab206874)
- Anti-PDGFR beta antibody [Y92] (Alexa Fluor® 568) (ab206875)
- Anti-PDGFR beta antibody [Y92] - Low endotoxin, Azide free (ab215978)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab32570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | 1/20. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| WB | 1/5000 - 1/20000. Predicted molecular weight: 124 kDa. For samples expressing low levels of PDGFR beta, the amount of lysate loaded may need to be increased to allow detection. |
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| IHC-P | 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Optimisation of the IHC protocol may be required depending on the sample used. |
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| ICC/IF | 1/100. | |
| IP | 1/20. | |
| IHC-FrFl | Use at an assay dependent concentration. PubMed: 25077029 |
Target
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FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
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Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 5159 Human
- Entrez Gene: 18596 Mouse
- Entrez Gene: 24629 Rat
- Omim: 173410 Human
- SwissProt: P09619 Human
- SwissProt: P05622 Mouse
- SwissProt: Q05030 Rat
- Unigene: 509067 Human
see all -
Alternative names
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-10-ab32570IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Immunohistochemical staining of paraffin embedded human spleen with purified ab32570 at a working dilution of 1/50. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-9-ab32570WB4.jpg)
Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)All lanes : Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/10000 dilution (purified)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/PDGF-Receptor-beta-Primary-antibodies-ab32570-9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Image from Miyata M et al., J Biol Chem. 2009 Sep 4;284(36):24595-609. Epub 2009 Jul 9. Fig 1.; doi: 10.1074/jbc.M109.016436; September 4, 2009, The Journal of Biological Chemistry, 284, 24595-24609.Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells stimulated with PDGF, staining PDGFR beta with unpurified ab32570.
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![Flow Cytometry - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-13-ab32570FC1.jpg)
Flow Cytometry - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Flow cytometry analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling PDGFR beta (red) with ab32570 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-304365-ab32570.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)ab32570 staining PDGFR beta in human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.
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![Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-6-ab32570WB1.jpg)
Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/10000 dilution (purified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-11-ab32570ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Immunofluorescence staining of NIH/3T3 (Mouse embryo fibroblast cell line) cells with purified ab32570 at a working dilution of 1 in 100, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.
The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab32570 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-304371-ab32570IHCHumbr.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)ab32570 staining PDGFR beta in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.
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![Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-8-ab32570WB3.jpg)
Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/50000 dilution (purified) + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-7-ab32570WB2.jpg)
Western blot - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 124 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)](https://www.abcam.com/ps/products/32/ab32570/Images/ab32570-12-ab32570IP1.jpg)
Immunoprecipitation - Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570)ab32570 (purified) at 1/20 immunoprecipitating PDGFR beta in NIH/3T3 (Mouse embryo fibroblast cell line) (Lane 1 and 2). Lane 3 - PBS.
For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
References
This product has been referenced in:
- Perin S et al. Isolation and characterisation of mouse intestinal mesoangioblasts. Pediatr Surg Int 35:29-34 (2019). Read more (PubMed: 30406837) »
- Greenhalgh SN et al. Loss of Integrin avß8 in Murine Hepatocytes Accelerates Liver Regeneration. Am J Pathol 189:258-271 (2019). Read more (PubMed: 30448409) »
