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Request you to please let me know whether abcam
can supply the following antibodies (reactivity:
1. Fluorescein isothiocyanate (FITC)-conjugated
antibody against the melanosome-specific protein
2. Phycoerythrin (PE)-conjugated antibody against
the keratinocyte-specific marker cytokeratin.
3. PE conjugated antibody against collagen 1, type
All are used for flowcytometry. Antibodies 1&2
will be used together
Asked on Feb 21 2012
Thank you for contacting us.
We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=52460).
For collagen I, we have only one unconjugated antibody that has been tested in flow cytometry. We do, however, have a kit that enables one to easily conjugate the antibody to PE.
Collagen I - ab23446
EasyLink PE - ab102919 or ab102918
For gp100, we have unconjugated antibodies but neither is in a formulation that is compatible with the conjugation kits for FITC. What I recommend is using the unconjugated anti-gp100, ab117853, followed by FITC-conjugated anti-mouse IgG2b, ab98702, pre-adsorbed to remove reactivity with mouse IgG1 (which is the isotype of ab52460, anti-cytokeratin). This should allow you to apply both antibodies to the same sample without cross-reaction of the secondary anti-IgG2b with mouse IgG1.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=117853).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=98702).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Abcam Scientific Support
Answered on Feb 21 2012