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I would like to use EasyLink for FITC or PE conjugation of my antibodies (I would like to use them for flow) but my antibodies concentration is lower than 1 mg/ml (between 0.1 and 0.5 mg/mL). Will it works? Do you have any previous experience?
Asked on Jun 25 2012
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The EasyLink conjugations require antibody concentrations of at least 1mg/ml. Below that, the conjugation becomes inefficient and you risk wasting the conjugate, not to mention the antibody. For antibodies of lower concentrations, we recommend bringing them up to 1mg/ml or more with a concentration columns. We have two sizes: one column or three columns.
Click here (or use the following: https://www.abcam.com/index.html?datasheet=102776).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=102778).
These are very easy to use, and ensure effective conjugations. Please let me know if you have any questions.
Abcam Scientific Support
Answered on Jun 25 2012