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Objective: I am interested in one of your R-PE conjugation kit for FACS antibodies.
Previously, we ordered Integrin B1 antibody ((12G10)(ab30394)) for FACS analysis.
Because your antibody is a purified one, I plan to conjugate R-PE dye to the antibodies and I found your R-PE conjugation kit (ab 102918).
I want to know whether I can use your R-PE conjugation kit-produced labeled antibodies directly to the sample
or I should use extra antibody purification step (such as column purification) before FACS analysis.
Please let me know ASAP.
Asked on Jan 31 2013
Thank you for contacting us.
We have the same integrin beta 1 antibody [12G10] that is FITC conjugated as ab150002, just so you know, but we do not have an R-PE conjugated version.
But if you would like to conjugate the ab30394 to R-PE using our EasyLink kit ab102918, that would definitely work. Once the antibody is conjugated using the kit, it is ready to use and no further purification is necessary.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Abcam Scientific Support
Answered on Jan 31 2013