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Question (71472) | PE / R-Phycoerythrin Conjugation Kit (ab102918)

Objective: I am interested in one of your R-PE conjugation kit for FACS antibodies.

Previously, we ordered Integrin B1 antibody ((12G10)(ab30394)) for FACS analysis.

Because your antibody is a purified one, I plan to conjugate R-PE dye to the antibodies and I found your R-PE conjugation kit (ab 102918).

I want to know whether I can use your R-PE conjugation kit-produced labeled antibodies directly to the sample
or I should use extra antibody purification step (such as column purification) before FACS analysis.

Please let me know ASAP.

Thank you for contacting us.

We have the same integrin beta 1 antibody [12G10] that is FITC conjugated as ab150002, just so you know, but we do not have an R-PE conjugated version.

But if you would like to conjugate the ab30394 to R-PE using our EasyLink kit ab102918, that would definitely work. Once the antibody is conjugated using the kit, it is ready to use and no further purification is necessary.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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