Anti-PEBP4 antibody (ab99468)
Key features and details
- Goat polyclonal to PEBP4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEBP4 antibody -
Description
Goat polyclonal to PEBP4 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PEBP4 aa 200-300 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_659399.2
Database link: Q96S96 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99468 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 26 kDa.
A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 26 kDa. A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. |
IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
PEBP4 appears to promote cellular resistance to TNF-induced apoptosis by inhibiting activation of the Raf-1/MEK/ERK pathway, JNK and phosphatidylethanolamine externalization. -
Cellular localization
Lysosome -
Database links
- Entrez Gene: 157310 Human
- Omim: 612473 Human
- SwissProt: Q96S96 Human
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Alternative names
- 1700051A02Rik antibody
- 1700081D17Rik antibody
- AI429082 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab99468 has been referenced in 2 publications.
- Li H et al. Phosphatidylethanolamine-binding protein 4 is associated with breast cancer metastasis through Src-mediated Akt tyrosine phosphorylation. Oncogene N/A:N/A (2013). IHC-P ; Human . PubMed: 24276246
- Yu GP et al. The expression of PEBP4 protein in lung squamous cell carcinoma. Tumour Biol 32:1257-63 (2011). WB, IHC-P ; Human . PubMed: 21887552