Recombinant Anti-PEDF antibody [EPR9826(B)] (ab157207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9826(B)] to PEDF
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PEDF antibody [EPR9826(B)]
See all PEDF primary antibodies -
Description
Rabbit monoclonal [EPR9826(B)] to PEDF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma; Human serum; Y79, A549, HEK-293T and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9826(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
Target
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Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Retinal pigment epithelial cells and blood plasma. -
Sequence similarities
Belongs to the serpin family. -
Developmental stage
Expressed in quiescent cells. -
Domain
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
Post-translational
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 5176 Human
- Omim: 172860 Human
- SwissProt: P36955 Human
- Unigene: 532768 Human
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Alternative names
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
Images
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All lanes : Anti-PEDF antibody [EPR9826(B)] (ab157207) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Y79 (Human retinoblastoma cell line) lysate
Lane 4 : A549 (Human lung carcinoma cell line) lysate
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 6 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab157207 has been referenced in 5 publications.
- Tian Y et al. Sonodynamic therapy suppresses matrix collagen degradation in vulnerable atherosclerotic plaque by modulating caspase 3 - PEDF/HIF-1α - MMP-2/MMP-9 signaling in macrophages. PLoS One 17:e0279191 (2022). PubMed: 36574366
- Ishida M et al. A ROCK Inhibitor Promotes Graft Survival during Transplantation of iPS-Cell-Derived Retinal Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33810153
- Janssen K et al. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling. Methods Mol Biol 1994:31-39 (2019). PubMed: 31124102
- Zhang R et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535:164-8 (2016). PubMed: 27383988
- Chen X et al. Metformin inhibits prostate cancer cell proliferation, migration, and tumor growth through upregulation of PEDF expression. Cancer Biol Ther 17:507-14 (2016). PubMed: 26987032