Anti-PER1 antibody - N-terminal (ab136451)
Key features and details
- Rabbit polyclonal to PER1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: n/a
Overview
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Product name
Anti-PER1 antibody - N-terminal
See all PER1 primary antibodies -
Description
Rabbit polyclonal to PER1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment:
EGADGGGDPRPGEPFC
, corresponding to N terminal amino acids 6-21 of Human PER1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500. Predicted molecular weight: 136 kDa.
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Notes |
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WB
1/500. Predicted molecular weight: 136 kDa. |
Target
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Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
Tissue specificity
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
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Database links
- Entrez Gene: 5187 Human
- Omim: 602260 Human
- SwissProt: O15534 Human
- Unigene: 445534 Human
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Alternative names
- Circadian clock protein PERIOD 1 antibody
- Circadian clock protein PERIOD1 antibody
- Circadian pacemaker protein Rigui antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab136451 has been referenced in 8 publications.
- Das M et al. Time-restricted feeding normalizes hyperinsulinemia to inhibit breast cancer in obese postmenopausal mouse models. Nat Commun 12:565 (2021). PubMed: 33495474
- de Assis LVM et al. Loss of Melanopsin (OPN4) Leads to a Faster Cell Cycle Progression and Growth in Murine Melanocytes. Curr Issues Mol Biol 43:1436-1450 (2021). PubMed: 34698095
- Levine DC et al. NAD+ Controls Circadian Reprogramming through PER2 Nuclear Translocation to Counter Aging. Mol Cell 78:835-849.e7 (2020). PubMed: 32369735
- Wang Z et al. Associated analysis of PER1/TUBB2B with endometrial cancer development caused by circadian rhythm disorders. Med Oncol 37:90 (2020). PubMed: 32926243
- Lu R et al. Necdin regulates BMAL1 stability and circadian clock through SGT1-HSP90 chaperone machinery. Nucleic Acids Res 48:7944-7957 (2020). PubMed: 32667666
- Yan L et al. Time-restricted Feeding Attenuates High-fat Diet-enhanced Spontaneous Metastasis of Lewis Lung Carcinoma in Mice. Anticancer Res 39:1739-1748 (2019). PubMed: 30952713
- Crosby P et al. Insulin/IGF-1 Drives PERIOD Synthesis to Entrain Circadian Rhythms with Feeding Time. Cell 177:896-909.e20 (2019). PubMed: 31030999
- Meier D et al. Twist1 Is a TNF-Inducible Inhibitor of Clock Mediated Activation of Period Genes. PLoS One 10:e0137229 (2015). WB . PubMed: 26361389