Anti-PERP antibody (ab5986)
Key features and details
- Rabbit polyclonal to PERP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PERP antibody
See all PERP primary antibodies -
Description
Rabbit polyclonal to PERP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide:
EDDLLGNAKPRYFYTSA
, corresponding to amino acids 175-193 of Human PERP. -
General notes
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 21 kDa.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 21 kDa. |
Target
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Function
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. -
Tissue specificity
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts. -
Sequence similarities
Belongs to the TMEM47 family. -
Cellular localization
Cell junction > desmosome. Cell membrane. Associated with desmosomes. - Information by UniProt
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Database links
- Entrez Gene: 64065 Human
- Entrez Gene: 64058 Mouse
- Omim: 609301 Human
- SwissProt: Q96FX8 Human
- SwissProt: Q9JK95 Mouse
- Unigene: 201446 Human
- Unigene: 28209 Mouse
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Alternative names
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
see all
Images
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All lanes : Anti-PERP antibody (ab5986) at 1 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K569 cell lysate
Lane 6 : MCF-7 cell lysate
Lane 7 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1
µ g /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml. -
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab5986 has been referenced in 10 publications.
- Liao CY et al. Novel function of PERP-428 variants impacts lung cancer risk through the differential regulation of PTEN/MDM2/p53-mediated antioxidant activity. Free Radic Biol Med 167:307-320 (2021). PubMed: 33731308
- Drucker A et al. Trastuzumab-induced upregulation of a protein set in extracellular vesicles emitted by ErbB2-positive breast cancer cells correlates with their trastuzumab sensitivity. Breast Cancer Res 22:105 (2020). PubMed: 33023655
- McDonnell SJ et al. ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions. Cell Death Discov 5:132 (2019). PubMed: 31508245
- Awais R et al. p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma. Br J Cancer 115:983-992 (2016). WB . PubMed: 27584665
- Khan IA et al. ErbB2-dependent downregulation of a pro-apoptotic protein Perp is required for oncogenic transformation of breast epithelial cells. Oncogene 35:5759-5769 (2016). PubMed: 27109096
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Hatanpaa KJ et al. High expression of the stem cell marker nestin is an adverse prognostic factor in WHO grade II-III astrocytomas and oligoastrocytomas. J Neurooncol 117:183-189 (2014). PubMed: 24519516
- Teh MT et al. A molecular study of desmosomes identifies a desmoglein isoform switch in head and neck squamous cell carcinoma. J Oral Pathol Med 40:67-76 (2011). WB ; Human . PubMed: 20923451
- Ding Z et al. Preferential gene expression in the limbus of the vervet monkey. Mol Vis 14:2031-41 (2008). IHC-Fr ; Monkey . PubMed: 18989386
- Paraoan L et al. Expression of p53-induced apoptosis effector PERP in primary uveal melanomas: downregulation is associated with aggressive type. Exp Eye Res 83:911-9 (2006). PubMed: 16784742