Anti-PF4 antibody [RTO] (ab49735)
Key features and details
- Mouse monoclonal [RTO] to PF4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PF4 antibody [RTO]
See all PF4 primary antibodies -
Description
Mouse monoclonal [RTO] to PF4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Recombinant full length protein corresponding to Human PF4.
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Positive control
- This antibody gave a positive signal in the following whole cell lysate: Human Platelet
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RTO -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab49735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 11 kDa.
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Notes |
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WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 11 kDa. |
Target
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Function
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsBinds non-covalently to a proteoglycan molecule. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5196 Human
- Entrez Gene: 360918 Rat
- Omim: 173460 Human
- SwissProt: P02776 Human
- SwissProt: P06765 Rat
- Unigene: 81564 Human
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Alternative names
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
Images
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Anti-PF4 antibody [RTO] (ab49735) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 20 minutes
Datasheets and documents
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Datasheet download
References (7)
ab49735 has been referenced in 7 publications.
- Loberg LI et al. Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeys. MAbs 13:1887628 (2021). PubMed: 33596779
- Wang X et al. Effects of CXCL4/CXCR3 on the lipopolysaccharide-induced injury in human umbilical vein endothelial cells. J Cell Physiol 234:22378-22385 (2019). PubMed: 31073998
- Zabini D et al. Angiostatic factors in the pulmonary endarterectomy material from chronic thromboembolic pulmonary hypertension patients cause endothelial dysfunction. PLoS One 7:e43793 (2012). IHC . PubMed: 22916307
- Li Y et al. Elevated expression of CXC chemokines in pediatric osteosarcoma patients. Cancer 117:207-17 (2011). IHC ; Human . PubMed: 20806347
- Shi L et al. Discovery and identification of potential biomarkers of pediatric Acute Lymphoblastic Leukemia. Proteome Sci 7:7 (2009). PubMed: 19291297
- Reilly MP et al. Heparin-induced thrombocytopenia/thrombosis in a transgenic mouse model requires human platelet factor 4 and platelet activation through FcgammaRIIA. Blood 98:2442-7 (2001). PubMed: 11588041
- Arepally GM et al. Characterization of a murine monoclonal antibody that mimics heparin-induced thrombocytopenia antibodies. Blood 95:1533-40 (2000). PubMed: 10688805