Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Bladder)
Specification
Bladder
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/3000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted image shows good strong immunostaining of intermediate nerve fibres ( and also larger fibres, not shown).
Small fibres are seen but are moderately positive, in my hands.
This Ab gives minimal background so could be used at a higher concentration.
Small fibres are seen but are moderately positive, in my hands.
This Ab gives minimal background so could be used at a higher concentration.
Mr. Carl Hobbs
Verified customer
Submitted Nov 29 2012