Anti-PGP9.5 antibody (ab72910)
Key features and details
- Chicken polyclonal to PGP9.5
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgY
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
Description
Chicken polyclonal to PGP9.5 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length human PGP9.5 purified from E. coli.
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Positive control
- Rat hippocampal homogenate and Rat mixed neuron/glial cultures.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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ICC/IF | (1) |
1/500.
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Notes |
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WB
1/2500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ICC/IF
1/500. |
Target
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Function
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
Tissue specificity
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
Involvement in disease
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
Sequence similarities
Belongs to the peptidase C12 family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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Database links
- Entrez Gene: 29545 Rat
- SwissProt: Q00981 Rat
- Unigene: 107213 Rat
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Alternative names
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab72910 has been referenced in 7 publications.
- Yu W et al. Role of P2X4Receptor in Mouse Voiding Function. Sci Rep 8:1838 (2018). ICC/IF . PubMed: 29382907
- Chen QQ et al. Age-dependent alpha-synuclein accumulation and aggregation in the colon of a transgenic mouse model of Parkinson's disease. Transl Neurodegener 7:13 (2018). PubMed: 29988485
- Wong R et al. Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neurons. Brain Behav Immun N/A:N/A (2018). PubMed: 30453020
- McQuade RM et al. Irinotecan-Induced Gastrointestinal Dysfunction Is Associated with Enteric Neuropathy, but Increased Numbers of Cholinergic Myenteric Neurons. Front Physiol 8:391 (2017). IHC-P ; Mouse . PubMed: 28642718
- Guan NN et al. Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2. Br J Pharmacol 172:4024-37 (2015). PubMed: 25917171
- Fu YY et al. 3-D imaging and illustration of mouse intestinal neurovascular complex. Am J Physiol Gastrointest Liver Physiol 304:G1-11 (2013). ICC/IF ; Mouse . PubMed: 23086917
- Allen C et al. Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA. J Immunol 189:381-92 (2012). PubMed: 22661091