Recombinant Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PHOX2B antibody [EPR14423] - C-terminal
See all PHOX2B primary antibodies -
Description
Rabbit monoclonal [EPR14423] to PHOX2B - C-terminal -
Host species
Rabbit -
Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Neuro-2a and SH-SY5Y cell lysate. IHC-P: Human neuroblastoma tissue. ICC/IF: HeLa, Neuro-2a and SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14423 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
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IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. -
Tissue specificity
Expressed in neuroblastoma, brain and adrenal gland. -
Involvement in disease
Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2) [MIM:613013]. A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8929 Human
- Entrez Gene: 18935 Mouse
- Omim: 603851 Human
- SwissProt: Q99453 Human
- SwissProt: O35690 Mouse
- Unigene: 87202 Human
- Unigene: 62505 Mouse
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Alternative names
- NBLST2 antibody
- NBPhox antibody
- Neuroblastoma paired type homeobox protein antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cell line labelling PHOX2B with ab183741 at 1/100 (10 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in Neuro-2a cell line.
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human neuroblastoma tissue labeling PHOX2B with ab183741 at 1/1000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-PHOX2B antibody [EPR14423] - C-terminal (ab183741) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa -
Immunofluorescence analysis of acetone-fixed HeLa cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).
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Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling PHOX2B with ab183741 at 1/100 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (11)
ab183741 has been referenced in 11 publications.
- Loh AHP et al. Pro-metastatic and mesenchymal gene expression signatures characterize circulating tumor cells of neuroblastoma patients with bone marrow metastases and relapse. Front Oncol 12:939460 (2022). PubMed: 36176417
- Le TL et al. Dysregulation of the NRG1/ERBB pathway causes a developmental disorder with gastrointestinal dysmotility in humans. J Clin Invest 131:N/A (2021). PubMed: 33497358
- Naveed A et al. NEAT1 polyA-modulating antisense oligonucleotides reveal opposing functions for both long non-coding RNA isoforms in neuroblastoma. Cell Mol Life Sci 78:2213-2230 (2021). PubMed: 32914209
- Yarmarkovich M et al. Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs. Nature 599:477-484 (2021). PubMed: 34732890
- Wu L et al. p50 mono-ubiquitination and interaction with BARD1 regulates cell cycle progression and maintains genome stability. Nat Commun 11:5007 (2020). PubMed: 33024116