Recombinant Anti-VPS34 antibody [EPR5301] (ab124905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5301] to VPS34
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-VPS34 antibody [EPR5301]
See all VPS34 primary antibodies -
Description
Rabbit monoclonal [EPR5301] to VPS34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human VPS34 aa 800-900. The exact sequence is proprietary.
-
Positive control
- L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.27 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5301 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. -
Tissue specificity
Ubiquitously expressed, with a highest expression in skeletal muscle. -
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
Cellular localization
Midbody. - Information by UniProt
-
Database links
- Entrez Gene: 5289 Human
- Entrez Gene: 225326 Mouse
- Entrez Gene: 65052 Rat
- Omim: 602609 Human
- SwissProt: Q8NEB9 Human
- SwissProt: Q6PF93 Mouse
- SwissProt: O88763 Rat
- Unigene: 464971 Human
see all -
Alternative names
- hVps34 antibody
- MGC61518 antibody
- Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
see all
Images
-
All lanes : Anti-VPS34 antibody [EPR5301] (ab124905) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : SHSY-5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab124905 has been referenced in 13 publications.
- Li L et al. Knockdown of miR-92a suppresses the stemness of colorectal cancer cells via mediating SOCS3. Bioengineered 13:5613-5624 (2022). WB ; Human . PubMed: 35184640
- Liu Y et al. TNF-a released from retinal Müller cells aggravates retinal pigment epithelium cell apoptosis by upregulating mitophagy during diabetic retinopathy. Biochem Biophys Res Commun 561:143-150 (2021). PubMed: 34023779
- Dai F et al. Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy. Front Pharmacol 12:705747 (2021). PubMed: 34483910
- Wang X et al. PIK3C3 Acts as a Tumor Suppressor in Esophageal Squamous Cell Carcinoma and Was Regulated by MiR-340-5p. Med Sci Monit 26:e920642 (2020). PubMed: 32207410
- Tan S et al. ß-Arrestin1 enhances liver fibrosis through autophagy-mediated Snail signaling. FASEB J 33:2000-2016 (2019). PubMed: 30216111