Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 alpha + Gamma
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase p85 alpha + Gamma antibody -
Description
Rabbit polyclonal to PI 3 Kinase p85 alpha + Gamma -
Host species
Rabbit -
Specificity
PI3 kinase p85 alpha and gamma. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic non-phosphopeptide derived from human PI 3 Kinase p85 alpha around the phosphorylation site of tyrosine 468 (R-L-YP-E-E).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF | (1) |
1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500. |
Target
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Relevance
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 5295 Human
- Entrez Gene: 8503 Human
- Omim: 171833 Human
- Omim: 606076 Human
- SwissProt: P27986 Human
- SwissProt: Q92569 Human
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Alternative names
- GRB1 antibody
- p55 antibody
- p55 GAMMA antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74136, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescence analysis of HeLa cells using ab74136, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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All lanes : Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with H2O2 (100µM, 30mins)
Lane 2 : Extracts from COS7 cells, treated
with H2O2 (100µM, 30mins) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
ab74136 at 1/200 dilution staining PI 3 Kinase p85 alpha + Gamma in mouse embryonic stem cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized in 1:1 methanol, acetone and blocked in 1% serum for 30 minutes at 25°C. The primary antibody was used at 1/100 dilution in Goat block dillution buffer and incubated with sample at 25°C for 1 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG (H+L) was used as secondary at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab74136 has been referenced in 12 publications.
- Liu W et al. Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice. Biosci Rep 40:N/A (2020). PubMed: 31904088
- Liu Z et al. Shenzhiling Oral Liquid Protects STZ-Injured Oligodendrocyte through PI3K/Akt-mTOR Pathway. Evid Based Complement Alternat Med 2020:4527283 (2020). PubMed: 32774416
- Wu S et al. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway. Evid Based Complement Alternat Med 2020:1403572 (2020). PubMed: 32802113
- Dou F et al. Aloe-Emodin Ameliorates Renal Fibrosis Via Inhibiting PI3K/Akt/mTOR Signaling Pathway In Vivo and In Vitro. Rejuvenation Res 22:218-229 (2019). PubMed: 30215298
- Xie L et al. Inhibiting the PI3K/AKT/NF-?B signal pathway with nobiletin for attenuating the development of osteoarthritis: in vitro and in vivo studies. Food Funct 10:2161-2175 (2019). PubMed: 30938722