Anti-PKC epsilon antibody (ab63638)
Key features and details
- Rabbit polyclonal to PKC epsilon
- Suitable for: ELISA, ICC/IF, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PKC epsilon antibody
See all PKC epsilon primary antibodies -
Description
Rabbit polyclonal to PKC epsilon -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic non phosphopeptide derived from human PKC epsilon around the phosphorylation site of serine 729 (G-F-SP-Y-F).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab63638 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration. PubMed: 25165884
|
|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 25165884 |
WB
1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
Target
-
Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsPhosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. - Information by UniProt
-
Database links
- Entrez Gene: 5581 Human
- Entrez Gene: 18754 Mouse
- Entrez Gene: 29340 Rat
- Omim: 176975 Human
- SwissProt: Q02156 Human
- SwissProt: P16054 Mouse
- SwissProt: P09216 Rat
- Unigene: 580351 Human
see all -
Alternative names
- KPCE_HUMAN antibody
- MGC125656 antibody
- MGC125657 antibody
see all
Images
-
Anti-PKC epsilon antibody (ab63638) at 1/500 dilution + Human Cell Lysate- Whole cell (HMEC) at 20 µg
Secondary
Donkey anti rabbit
Conjugation: HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Exposure time: 10 minutes -
Anti-PKC epsilon antibody (ab63638) at 1/500 dilution + Mouse Cell lysate - whole cell (NIH3T3)
at 20 µg
Secondary
Donkey anti rabbit HRP linked
Conjugation: HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Exposure time: 10 minutes
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab63638 has been referenced in 8 publications.
- Xu J et al. Oxidative stress induced by NOX2 contributes to neuropathic pain via plasma membrane translocation of PKCe in rat dorsal root ganglion neurons. J Neuroinflammation 18:106 (2021). PubMed: 33952299
- Wang M et al. Morphine post-conditioning-induced up-regulation of lncRNA TINCR protects cardiomyocytes from ischemia-reperfusion injury via inhibiting degradation and ubiquitination of FGF1. QJM 113:859-869 (2020). PubMed: 32176291
- Inprasit C et al. Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model. Int J Mol Med 45:779-792 (2020). PubMed: 31922226
- Koundouros N et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell 181:1596-1611.e27 (2020). PubMed: 32559461
- El Bezawy R et al. miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCe and ZEB1 inhibition. J Exp Clin Cancer Res 38:51 (2019). PubMed: 30717752
- Zhang J et al. Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGK? gene knockout. Mol Med Rep 17:2169-2176 (2018). PubMed: 29207074
- Lin YH et al. Cyclic mechanical strain of myocytes modifies CapZß1 post translationally via PKCe. J Muscle Res Cell Motil 36:329-37 (2015). PubMed: 26429793
- Foerster F et al. Targeting the actin cytoskeleton: selective antitumor action via trapping PKC?. Cell Death Dis 5:e1398 (2014). IHC-P, IHC-Fr . PubMed: 25165884