Recombinant Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC iota (phospho T412)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] -
Description
Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC iota (phospho T412) -
Host species
Rabbit -
Specificity
This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.
This antibody also detects various PKC phosphorylated isoforms.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293 cell lysate, human brain tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1491Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/20000. Predicted molecular weight: 68 kDa.
For unpurified, use 1/1000 - 1/2000. |
Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/20000. Predicted molecular weight: 68 kDa. For unpurified, use 1/1000 - 1/2000. |
Target
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Cellular localization
PKC zeta: Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. PKC iota: Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A. -
Database links
- Entrez Gene: 5584 Human
- Entrez Gene: 5590 Human
- Entrez Gene: 18759 Mouse
- Entrez Gene: 18762 Mouse
- Entrez Gene: 25522 Rat
- Entrez Gene: 84006 Rat
- Omim: 176982 Human
- Omim: 600539 Human
see all
Images
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All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/1000 dilution
Lane 1 :Recombinant human PKC alpha protein (Active) (ab55672)
Lane 2 :Recombinant human PKC beta 1 protein (ab60840)
Lane 3 :Recombinant human PKC beta 2 protein (ab60841)
Lane 4 :Recombinant human PKC gamma protein (ab60842)
Lane 5 :Recombinant human PKC delta protein (ab60844)
Lane 6 :Recombinant human PKC epsilon protein (ab60847)
Lane 7 :Recombinant human PKC zeta protein (ab60848)
Lane 8 :Recombinant human PKC eta protein (ab60849)
Lane 9 :Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 10 :Recombinant human PKC iota protein (ab60850)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
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Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified) + Mouse lung tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution (unpurified)
Lane 1 : 293 cell lysates, untreated
Lane 2 : 293 cell lysates, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit-HRP at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 165 kDa, 170 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified)
Lane 1 : Untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab76129 has been referenced in 4 publications.
- Liu S et al. Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation. Front Nutr 9:853225 (2022). PubMed: 35356725
- Chau DD et al. Attenuation of amyloid-β generation by atypical protein kinase C-mediated phosphorylation of engulfment adaptor PTB domain containing 1 threonine 35. FASEB J 33:12019-12035 (2019). PubMed: 31373844
- Yeo MK et al. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J Cancer 8:3371-3377 (2017). PubMed: 29158810
- Raychaudhuri S MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-ß Factor SMAD4. PLoS One 7:e34596 (2012). WB ; Rat . PubMed: 22506032