Overview

  • Product name

    Anti-PLAGL1 / ZAC antibody
    See all PLAGL1 / ZAC primary antibodies
  • Description

    Rabbit polyclonal to PLAGL1 / ZAC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 72-121 (NRKDHLKNHL QTHDPNKMAF GCEECGKKYN TMLGYKRHLA LHAASSGDLT) of Human PLAGL1/ ZAC (NP_006709)

  • Positive control

    • Human fetal kidney lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp781P1017 antibody
    • Lost on transformation 1 antibody
    • Lost on Transformation antibody
    • LOT-1 antibody
    • LOT1 antibody
    • MGC126275 antibody
    • MGC126276 antibody
    • PLAG like 1 antibody
    • PLAGL1 antibody
    • PLAL1_HUMAN antibody
    • Pleiomorphic adenoma gene like 1 antibody
    • Pleiomorphic adenoma gene like protein 1 antibody
    • Pleiomorphic adenoma-like protein 1 antibody
    • Tumor supressor ZAC antibody
    • ZAC antibody
    • ZAC tumor supressor antibody
    • ZAC1 antibody
    • Zinc finger protein PLAGL1 antibody
    see all

Images

  • Anti-PLAGL1 / ZAC antibody (ab90472) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa
    Additional bands at: <22 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12%

References

This product has been referenced in:

  • Cai F  et al. A Novel Mutation of Aryl Hydrocarbon Receptor Interacting Protein Gene Associated with Familial Isolated Pituitary Adenoma Mediates Tumor Invasion and Growth Hormone Hypersecretion. World Neurosurg N/A:N/A (2018). Read more (PubMed: 30447469) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Mouse Tissue lysate - nuclear (Placenta)
Total protein in input
100 µg
Immuno-precipitation step
Protein A/G
Specification
Placenta

Abcam user community

Verified customer

Submitted Feb 22 2019

Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Placenta)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 26 2017

Abreviews
Application
ChIP
Sample
Cow Tissue lysate - whole (Day ~105 fetal heart and kidney)
Negative control
PCR negative control: non-template control ChIP negative control: protein A/G magnetic beads ChIP negative control regions: IGF2 intron1 and GAPDH exon1* *Note: PLAGL1 has not been reported to bind to these two regions in mice and human. Particularly, it has been shown that PLAGL1 does not bind to IGF2 in mouse (Varrault et al., 2006 Dev Cell). In addition, the PLAGL1 binding motif in mouse GLUT4 (and putative motif in cow) was not found in IGF2 intron1 and GAPDH exon1 in cow.
Specification
Day ~105 fetal heart and kidney
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde (Sigma F8775)
Positive control
PCR positive control: genomic DNA (bovine fetal heart) Chromatin input: 1% of the chromatin used for immunoprecipitation ChIP positive control region: GLUT4 promoter region* *Note: PLAGL1 has been reported to bind to GLUT4 promoter region in mice (Czubryt MP et al., 2010 Journal of Biological Chemistry). The PLAGL1 binding motif in mouse GLUT4 is conserved in cow.

Abcam user community

Verified customer

Submitted Apr 20 2015

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