Recombinant Anti-PMCA1 antibody [EPR12029] (ab190355)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12029] to PMCA1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PMCA1 antibody [EPR12029]
See all PMCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12029] to PMCA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1, HeLa, Neuro-2a, U87-MG, HepG2, C6 cell lysates. IHC-P: Human stomach and Human transitional cell carcinoma of bladder tissues, mouse lung, mouse stomach, rat stomach.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12029 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
Tissue specificity
Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
Domain
The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P11505 Rat
- Unigene: 506276 Human
- Unigene: 7208 Rat
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Alternative names
- AT2B1_HUMAN antibody
- ATP2B1 antibody
- ATPase Ca++ transporting plasma membrane 1 antibody
see all
Images
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/5000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, boiled
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, unboiled
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, boiled
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate, unboiled
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate, boiled
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lanes 1, 2: 7 seconds
Lanes 3, 4, 5, 6: 3 secondsab181602 is used as a Loading Control at dilution 1/1000000.
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Immunohistochemical analysis of paraffin-embedded rat stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATP2B1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 180-245 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control ab8245 observed at 36 kDa.
ab190355 Anti-PMCA1 antibody [EPR12029] was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265561 (knockout cell lysate ab257365) was used. Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE. ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse lung cancer.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labelling PMCA1 with ab190355 at 1/2000 dilution, followed by ready-to-use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at dilution 1/1. Counterstained with Hematoxylin.
Performed heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining mouse stomach.
The section was incubated with ab190355 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab190355 has been referenced in 10 publications.
- Rodrat M et al. Vasoactive intestinal peptide and cystic fibrosis transmembrane conductance regulator contribute to the transepithelial calcium transport across intestinal epithelium-like Caco-2 monolayer. PLoS One 17:e0277096 (2022). PubMed: 36399482
- Ilic K et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. Int J Mol Sci 22:N/A (2021). PubMed: 34948386
- Dharmaraj P et al. Neonatal Hypocalcemic Seizures in Offspring of a Mother With Familial Hypocalciuric Hypercalcemia Type 1 (FHH1). J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32150253
- Bathala P et al. Oviductal extracellular vesicles (oviductosomes, OVS) are conserved in humans: murine OVS play a pivotal role in sperm capacitation and fertility. Mol Hum Reprod 24:143-157 (2018). PubMed: 29370405
- Fereshteh Z et al. Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility. J Cell Physiol N/A:N/A (2018). PubMed: 30536802
- Gorvin CM et al. A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate ß-arrestin-biased signaling. Sci Signal 11:N/A (2018). PubMed: 29463778
- Gorvin CM et al. Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias. Hum Mol Genet 27:3720-3733 (2018). PubMed: 30052933
- Frandsen SK et al. Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation. Cancer Res 77:4389-4401 (2017). PubMed: 28760856
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
- Chu W et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). PubMed: 27602089