• Product name
  • Description
    Rabbit polyclonal to PNPT1
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1-753 of Human PNPT1.

  • Positive control
    • Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. A431 whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human testis seminoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCo2.



Our Abpromise guarantee covers the use of ab96176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance
    PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain.
  • Cellular localization
  • Database links
  • Alternative names
    • 3' 5' RNA exonuclease antibody
    • COXPD13 antibody
    • DFNB70 antibody
    • old-35 antibody
    • OLD35 antibody
    • PNPASE antibody
    • PNPase 1 antibody
    • Polynucleotide phosphorylase like protein antibody
    • Polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody
    see all


  • ICC/IF image of ab96176 stained CaC02 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96176 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of PNPT1 staining in Human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96176, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PNPT1 antibody (ab96176) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 86 kDa


This product has been referenced in:
  • Dhir A  et al. Mitochondrial double-stranded RNA triggers antiviral signalling in humans. Nature 560:238-242 (2018). Read more (PubMed: 30046113) »
  • Toompuu M  et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). Read more (PubMed: 29518244) »
See all 2 Publications for this product

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