Anti-PNPT1 antibody (ab96176)
Key features and details
- Rabbit polyclonal to PNPT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNPT1 antibody
See all PNPT1 primary antibodies -
Description
Rabbit polyclonal to PNPT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 1-753 of Human PNPT1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 86 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 86 kDa. |
Target
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Relevance
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain. -
Cellular localization
Mitochondrion -
Database links
- Entrez Gene: 87178 Human
- GenBank: NP_149100.2 Human
- Omim: 610316 Human
- SwissProt: Q8TCS8 Human
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Alternative names
- 3' 5' RNA exonuclease antibody
- COXPD13 antibody
- DFNB70 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab96176 has been referenced in 8 publications.
- van Esveld SL et al. Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome. J Inherit Metab Dis 45:292-307 (2022). PubMed: 35023579
- Barbier M et al. Heterozygous PNPT1 Variants Cause Spinocerebellar Ataxia Type 25. Ann Neurol 92:122-137 (2022). PubMed: 35411967
- Hu QG et al. MiR-183-5p-PNPT1 Axis Enhances Cisplatin-induced Apoptosis in Bladder Cancer Cells. Curr Med Sci 42:785-796 (2022). PubMed: 35788944
- Coomans de Brachène A et al. Endogenous mitochondrial double-stranded RNA is not an activator of the type I interferon response in human pancreatic beta cells. Auto Immun Highlights 12:6 (2021). PubMed: 33773604
- Rius R et al. Clinical Spectrum and Functional Consequences Associated with Bi-Allelic Pathogenic PNPT1 Variants. J Clin Med 8:N/A (2019). PubMed: 31752325
- Dhir A et al. Mitochondrial double-stranded RNA triggers antiviral signalling in humans. Nature 560:238-242 (2018). PubMed: 30046113
- Toompuu M et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). PubMed: 29518244
- Liu X et al. PNPT1 Release from Mitochondria during Apoptosis Triggers Decay of Poly(A) RNAs. Cell 174:187-201.e12 (2018). PubMed: 29779946