Anti-POLRMT antibody (ab32988)
Key features and details
- Rabbit polyclonal to POLRMT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLRMT antibody
See all POLRMT primary antibodies -
Description
Rabbit polyclonal to POLRMT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human POLRMT aa 1200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32987) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates; HeLa, HEK293 and Caco2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa).
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Notes |
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WB
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 139 kDa). |
Target
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Function
DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. -
Sequence similarities
Belongs to the phage and mitochondrial RNA polymerase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 5442 Human
- Entrez Gene: 216151 Mouse
- Omim: 601778 Human
- SwissProt: O00411 Human
- SwissProt: Q8BKF1 Mouse
- Unigene: 254113 Human
- Unigene: 39001 Mouse
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Alternative names
- APOLMT antibody
- DNA-directed RNA polymerase antibody
- DNA-directed RNA polymerase mitochondrial antibody
see all
Images
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All lanes : Anti-POLRMT antibody (ab32988) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 139 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 45 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab32988 has been referenced in 11 publications.
- Kozhukhar N et al. A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication. Cells 11:N/A (2022). PubMed: 35883613
- Hangas A et al. Top3α is the replicative topoisomerase in mitochondrial DNA replication. Nucleic Acids Res 50:8733-8748 (2022). PubMed: 35904803
- Zhu Z et al. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria. Free Radic Biol Med 141:159-171 (2019). PubMed: 31212063
- van Esveld SL et al. A Combined Mass Spectrometry and Data Integration Approach to Predict the Mitochondrial Poly(A) RNA Interacting Proteome. Front Cell Dev Biol 7:283 (2019). PubMed: 31803741
- Kim Y et al. PKR Senses Nuclear and Mitochondrial Signals by Interacting with Endogenous Double-Stranded RNAs. Mol Cell 71:1051-1063.e6 (2018). PubMed: 30174290