Anti-Brn-2 antibody (ab101726)
Key features and details
- Goat polyclonal to Brn-2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Brn-2 antibody
See all Brn-2 primary antibodies -
Description
Goat polyclonal to Brn-2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Cow, Pig -
Immunogen
Synthetic peptide corresponding to Human Brn-2 aa 37-52 (internal sequence).
Sequence:C-AQSLVQGDYGALQSN
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Positive control
- WB: Human brain (cerebellum), mouse and rat brain tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. -
Tissue specificity
Expressed specifically in the neuroectodermal cell lineage. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 783320 Cow
- Entrez Gene: 18992 Mouse
- SwissProt: P31360 Mouse
- Unigene: 389520 Mouse
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Alternative names
- Brain 2 antibody
- Brain specific homeobox/POU domain protein 2 antibody
- Brain-2 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (3)
ab101726 has been referenced in 3 publications.
- Lowenstein ED et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268
- Ishii J et al. POU domain transcription factor BRN2 is crucial for expression of ASCL1, ND1 and neuroendocrine marker molecules and cell growth in small cell lung cancer. Pathol Int 63:158-68 (2013). PubMed: 23530560
- Sakaeda M et al. Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer. Lab Invest : (2013). PubMed: 23358112