Anti-PP2A-alpha antibody (ab137825)
Key features and details
- Rabbit polyclonal to PP2A-alpha
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PP2A-alpha antibody
See all PP2A-alpha primary antibodies -
Description
Rabbit polyclonal to PP2A-alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Recombinant fragment aa 1-231.
Database link: P67775 -
Positive control
- HeLa, HepG2, Molt 4 or Mouse brain whole lysates
Cal27 xenograft
Methanol fixed Hela cells
- HeLa, HepG2, Molt 4 or Mouse brain whole lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
Target
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 416318 Chicken
- Entrez Gene: 282320 Cow
- Entrez Gene: 403608 Dog
- Entrez Gene: 5515 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 397656 Pig
- Entrez Gene: 100009252 Rabbit
- Entrez Gene: 24672 Rat
see all -
Alternative names
- PP2A alpha antibody
- PP2A-alpha antibody
- PP2AA_HUMAN antibody
see all
Images
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Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 36 kDa
10% SDS PAGE -
Anti-PP2A-alpha antibody (ab137825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 36 kDa -
Confocal immunofluorescence analysis of methanol fixed HeLa cells, labelling PP2A alpha with ab137825 (Green) at 1/500 dilution. Alpha tubulin filaments were labelled with an alpha tubulin antibody (Red) at 1/2000.
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Immunohistochemical analysis of paraffin embedded Cal27 xenograft, labelling PP2A alpha with ab137825 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137825 has been referenced in 4 publications.
- Gu L et al. Inhibitory Effects of Macelignan on Tau Phosphorylation and Aβ Aggregation in the Cell Model of Alzheimer's Disease. Front Nutr 9:892558 (2022). PubMed: 35662922
- Plano SA et al. Role of G-Substrate in the NO/cGMP/PKG Signal Transduction Pathway for Photic Entrainment of the Hamster Circadian Clock. ASN Neuro 13:1759091420984920 (2021). PubMed: 33430619
- Xie Y et al. The proteasome activator REG? accelerates cardiac hypertrophy by declining PP2Aca-SOD2 pathway. Cell Death Differ N/A:N/A (2020). PubMed: 32424140
- Cao GP et al. Anxiolytic and neuroprotective effects of the Traditional Chinese Medicinal formulation Dan-zhi-xiao-yao-san in a rat model of chronic stress. Mol Med Rep 14:1247-54 (2016). PubMed: 27279479