Anti-PPHLN1/Periphilin-1 antibody (ab69569)
Key features and details
- Rabbit polyclonal to PPHLN1/Periphilin-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPHLN1/Periphilin-1 antibody -
Description
Rabbit polyclonal to PPHLN1/Periphilin-1 -
Host species
Rabbit -
Specificity
ab69569 detects endogenous levels of total PPHLN protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Extracts from COLO or 293 cells. Liver carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation. -
Tissue specificity
Ubiquitous. -
Post-translational
modificationsSubstrate of transglutaminase (in vitro). -
Cellular localization
Nucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions. - Information by UniProt
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Database links
- Entrez Gene: 51535 Human
- Entrez Gene: 223828 Mouse
- Omim: 608150 Human
- SwissProt: Q8NEY8 Human
- SwissProt: Q8K2H1 Mouse
- Unigene: 444157 Human
- Unigene: 74208 Mouse
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Alternative names
- Gastric cancer antigen Ga50 antibody
- Periphilin 1 antibody
- Periphilin-1 antibody
see all
Images
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All lanes : Anti-PPHLN1/Periphilin-1 antibody (ab69569) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 100 kDa, 35 kDa, 40 kDa, 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab69569 has been referenced in 4 publications.
- Das A et al. Epigenetic Silencing of Recombinant Adeno-associated Virus Genomes by NP220 and the HUSH Complex. J Virol 96:e0203921 (2022). PubMed: 34878926
- Douse CH et al. TASOR is a pseudo-PARP that directs HUSH complex assembly and epigenetic transposon control. Nat Commun 11:4940 (2020). PubMed: 33009411
- Zhang F et al. Characterization of a core region in the A2UCOE that confers effective anti-silencing activity. Sci Rep 7:10213 (2017). PubMed: 28860464
- Martrat G et al. Exploring the link between MORF4L1 and risk of breast cancer. Breast Cancer Res 13:R40 (2011). PubMed: 21466675