Recombinant Anti-PPP1R11 antibody [EPR11965] (ab171960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11965] to PPP1R11
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPP1R11 antibody [EPR11965]
See all PPP1R11 primary antibodies -
Description
Rabbit monoclonal [EPR11965] to PPP1R11 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis, HeLa, fetal brain, NIH: OVCAR-3 and MDA-MB-435 lysates. Permeabilized HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11965 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IP |
1/10 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IP
1/10 - 1/100. |
Target
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Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 6992 Human
- Entrez Gene: 76497 Mouse
- Entrez Gene: 294207 Rat
- Omim: 606670 Human
- SwissProt: O60927 Human
- SwissProt: Q8K1L5 Mouse
- SwissProt: Q6MFY6 Rat
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Alternative names
- HCG V antibody
- HCG-V antibody
- HCGV antibody
see all
Images
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All lanes : Anti-PPP1R11 antibody [EPR11965] (ab171960) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain tissue lysate
Lane 4 : NIH:OVCAR-3 cell lysate
Lane 5 : MDA-MB-435 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling PPP1R11 with ab171960 at 1/10 dilution (red)or a Rabbit IgG (negative) (green).
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Western blot analysis on immunoprecipitation pellet from Human testis lysate (1) labeling PPP1R11 with ab171960 at 1/10 dilution or 1X PBS (2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171960 has been referenced in 1 publication.
- Matsumoto Y et al. Tankyrase represses autoinflammation through the attenuation of TLR2 signaling. J Clin Invest 132:N/A (2022). PubMed: 35362478