Recombinant Anti-PPWD1 antibody [EPR7440] (ab126710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7440] to PPWD1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPWD1 antibody [EPR7440]
See all PPWD1 primary antibodies -
Description
Rabbit monoclonal [EPR7440] to PPWD1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, A549, HeLa, L929, L6, C6, and NIH 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7440 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 4 WD repeats. -
Cellular localization
Nucleus. Associated with spliceosomal complexes. - Information by UniProt
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Database links
- Entrez Gene: 23398 Human
- Entrez Gene: 238831 Mouse
- Entrez Gene: 294711 Rat
- SwissProt: Q96BP3 Human
- SwissProt: Q8CEC6 Mouse
- Unigene: 121432 Human
- Unigene: 98910 Mouse
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Alternative names
- 4632422M10Rik antibody
- A330090G21Rik antibody
- KIAA0073 antibody
see all
Images
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All lanes : Anti-PPWD1 antibody [EPR7440] (ab126710) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : L929 cell lysate
Lane 5 : L6 cell lysate
Lane 6 : C6 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126710 has been referenced in 1 publication.
- Admoni-Elisha L et al. Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment. PLoS One 11:e0148500 (2016). Human . PubMed: 27078856