Anti-PRAF1 antibody (ab88623)
Key features and details
- Mouse polyclonal to PRAF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PRAF1 antibody -
Description
Mouse polyclonal to PRAF1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PRAF1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- U-2 OS cell lysate, PRAF1 transfected 293T cell lysate and HeLa cell
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. -
Sequence similarities
Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 64425 Human
- SwissProt: Q9GZS1 Human
- Unigene: 591087 Human
-
Alternative names
- A49 antibody
- DNA directed RNA polymerase I subunit RPA49 antibody
- DNA-directed RNA polymerase I subunit E antibody
see all
Images
-
Anti-PRAF1 antibody (ab88623) at 1 µg/ml + U-2 OS at 50 µg
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-PRAF1 antibody (ab88623) at 1 µg/ml
Lane 1 : PRAF1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunofluorescence of ab88623 on HeLa cell (antibody concentration 10 ug/ml)
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab88623 has not yet been referenced specifically in any publications.