Overview

  • Product name
  • Description
    Rabbit polyclonal to PRDM14
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, ChIP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PRDM14.
    Database link: Q9GZV8

  • Positive control
    • BT474, Jurkat and HeLa cell extracts;

Properties

Applications

Our Abpromise guarantee covers the use of ab187881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP 1/20 - 1/100.
ChIP 1/20 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.
IHC-P 1/50 - 1/200.

Target

Images

  • Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab187881. Blue DAPI for nuclear staining.
  • All lanes : Anti-PRDM14 antibody (ab187881) at 1/500 dilution

    Lane 1 : BT474 cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : HeLa cell extract

    Predicted band size: 64 kDa

  • All lanes : Anti-PRDM14 antibody (ab187881) at 1/500 dilution

    Lane 1 : shRNA-6
    Lane 2 : Control
    Lane 3 : shRNA-5
    Lane 4 : shRNA-4
    Lane 5 : shRNA-3
    Lane 6 : shRNA-2
    Lane 7 : shRNA-1

    Predicted band size: 64 kDa



    Western blot analysis of various groups from PA-1 cell line. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.

  • Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using ab187881 and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

References

This product has been referenced in:
  • Gell JJ  et al. PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res 27:46-56 (2018). WB, ICC/IF . Read more (PubMed: 29324254) »
  • Moriya C  et al. PRDM14 directly interacts with heat shock proteins HSP90a and glucose-regulated protein 78. Cancer Sci 109:373-383 (2018). Read more (PubMed: 29178343) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 0)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 0
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 05 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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