Overview

  • Product name

  • Description

    Rabbit polyclonal to PRDM16
  • Host species

    Rabbit
  • Specificity

    At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid peptide from near the carboxy terminus of Human PRDM16 (CAH71530).

  • Positive control

    • WB: K562 cell lysate; Rat brain tissue lysate; Mouse MEL cell lysate. IHC-P: Human and rat brain tissue. ICC/IF: Human brown adipocytes from supraclavicular fat tissue; Human brain cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab106410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.

Target

  • Function

    Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
  • Tissue specificity

    Expressed in uterus and kidney.
  • Involvement in disease

    Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia.
  • Sequence similarities

    Contains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMD1LL antibody
    • KIAA1675 antibody
    • LVNC8 antibody
    • MDS1/EVI1 like gene 1 antibody
    • MDS1/EVI1-like gene 1 antibody
    • MEL1 antibody
    • PFM 13 antibody
    • PFM13 antibody
    • PR domain containing 16 antibody
    • PR domain containing protein 16 antibody
    • PR domain zinc finger protein 16 antibody
    • PR domain-containing protein 16 antibody
    • PRD16_HUMAN antibody
    • Prdm16 antibody
    • Transcription factor MEL 1 antibody
    • Transcription factor MEL1 antibody
    see all

Images

  • Anti-PRDM16 antibody (ab106410) at 1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Predicted band size: 140 kDa

  • Paraffin-embedded human brain tissue stained for PRDM16 using ab106410 at 2 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-PRDM16 antibody (ab106410) at 1 µg/ml
    Lane 2 : Anti-PRDM16 antibody (ab106410) at 2 µg/ml

    All lanes : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 140 kDa

  • ab106410 at 2.5 µg/ml, staining PRDM16 in paraffin embedded rat brain tissue.

  • ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-PRDM16 antibody (ab106410) at 1/2500 dilution (12 hours at 4°C, 1% skim milk) + Mouse MEL whole cell lysate at 17 µg with Milk, 1 hour at 25°C at 5 %

    Secondary
    HRP anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 140 kDa
    Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

    See Abreview

  • Immunofluorescence of PRDM16 in Human Brain cells using ab106410 at 20 ug/ml.

References

This product has been referenced in:

  • Cunarro J  et al. p107 Deficiency Increases Energy Expenditure by Inducing Brown-Fat Thermogenesis and Browning of White Adipose Tissue. Mol Nutr Food Res 63:e1801096 (2019). Read more (PubMed: 30383332) »
  • Wang H  et al. Phytanic acid activates PPARa to promote beige adipogenic differentiation of preadipocytes. J Nutr Biochem 67:201-211 (2019). Read more (PubMed: 30951974) »
See all 28 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Application
Western blot
Loading amount
17 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (MEL)
Specification
MEL
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (endothelial cell)
Specification
endothelial cell
Permeabilization
Yes - tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
10% serum,1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (Brown adipocytes from supraclavicular fat tissue)
Specification
Brown adipocytes from supraclavicular fat tissue
Permeabilization
Yes - 0.3% Triton X100
Fixative
Paraformaldehyde

Mrs. Lena Appelsved

Verified customer

Submitted Nov 21 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 21 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (tadpole limb)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - 1% Triton X-100 10minutes
Specification
tadpole limb
Blocking step
Roche blocking reagent 2% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde

Gufa Lin

Verified customer

Submitted Dec 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human ductal carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization
Yes - tween-20
Specification
Human ductal carcinoma
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 11 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample
Mouse Tissue sections (Lymph node)
Specification
Lymph node
Permeabilization
Yes - tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 29 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10.5%)
Sample
Mouse Tissue lysate - other (Brown adipose tissue)
Specification
Brown adipose tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 12 2013

Answer

Thank you for contacting us.

I have contacted the originating lab on your behalf. While the exact sequence is proprietary, but the immunogen is from aa1101-1160 of Human PRDM16 (CAH71530).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



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