Anti-PRDM16 antibody (ab85874)
Key features and details
- Rabbit polyclonal to PRDM16
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRDM16 antibody
See all PRDM16 primary antibodies -
Description
Rabbit polyclonal to PRDM16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PRDM16 aa 1250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab95842) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 140 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 140 kDa). |
Target
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Function
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation. -
Tissue specificity
Expressed in uterus and kidney. -
Involvement in disease
Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. -
Sequence similarities
Contains 10 C2H2-type zinc fingers.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 63976 Human
- Omim: 605557 Human
- SwissProt: Q9HAZ2 Human
- Unigene: 99500 Human
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Alternative names
- CMD1LL antibody
- KIAA1675 antibody
- LVNC8 antibody
see all
Images
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All lanes : Anti-PRDM16 antibody (ab85874) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85874 has not yet been referenced specifically in any publications.