Recombinant Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free (ab249281)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9432] to PRDM4/PFM1 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PRDM4/PFM1 antibody [EPR9432] - BSA and Azide free
See all PRDM4/PFM1 primary antibodies -
Description
Rabbit monoclonal [EPR9432] to PRDM4/PFM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249281 is the carrier-free version of ab156867.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9432 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 88 kDa. |
Target
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Function
May function as a transcription factor involved in cell differentiation. -
Tissue specificity
Expressed in many tissues. Highly expressed in ovary, testis, pancreas, brain, heart and prostate. -
Sequence similarities
Contains 6 C2H2-type zinc fingers.
Contains 1 SET domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11108 Human
- Entrez Gene: 72843 Mouse
- Entrez Gene: 170820 Rat
- Omim: 605780 Human
- SwissProt: Q9UKN5 Human
- SwissProt: Q80V63 Mouse
- SwissProt: Q9QZP2 Rat
- Unigene: 506655 Human
see all -
Alternative names
- MGC45046 antibody
- PFM 1 antibody
- PFM1 antibody
see all
Images
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This data was developed using ab156867, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaThis data was developed using ab156867, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H + L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaThis data was developed using ab156867, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab156867, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
This data was developed using ab156867, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
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This data was developed using ab156867, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of 293 cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249281 has not yet been referenced specifically in any publications.